Strain Data Sheet

RBRC02382

Strain Information

Image
BRC No.RBRC02382
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CXCL14)20
Former Common namehBRAKlongExp20L, C57BL/6J-Tg(CXCL14)1
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearance
Strain developmentDeveloped by Ryu-Ichiro Hata, Kanagawa Dental College in 2007.
Strain descriptionThis transgenic mouse expresses the human chemokine (C-X-C motif) ligand 14 gene under he control of CAG promoter. line 20. Level of CXCL14/BRAK is 14 ng/ml plasma (in homozygote).
Colony maintenance
References
BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells.
Ozawa S, Kato Y, Komori R, Maehata Y, Kubota E, Hata R
Biochem. Biophys. Res. Commun., 348, 406-412 (2006). 16884687

Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. [corrected]
Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata R
Transgenic Res., 19, 1109-1117 (2010). 20333465

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CXCL14 chemokine (C-X-C motif) ligand 14UNCXCL14 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
poly A polyAUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal tumor pathology(MP:0010639)

  • decreased metastatic potential(MP:0001273)

  • decreased tumor growth/size(MP:0003447)

  • decreased tumor incidence(MP:0002052)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Human BRAKLong cDNA, SV40Late polyA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorRyu-Ichiro Hata (Kanagawa Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ishida Y, Kimura A, Nosaka M, Kuninaka Y, Hemmi H, Sasaki I, Kaisho T, Mukaida N, Kondo T.
    Essential involvement of the CX3CL1-CX3CR1 axis in bleomycin-induced pulmonary fibrosis via regulation of fibrocyte and M2 macrophage migration.
    Sci Rep 7(1) 16833(2017) 29203799