Strain Data Sheet

RBRC02385

Strain Information

Image
BRC No.RBRC02385
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Rps6kb1<tm1Nte>
Former Common namep70s6 Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Hideki Kawasome, Natonal Jewish Medical and Research Center in 1997.
Strain descriptionHomozygous mutant mice are small.
Colony maintenance
References
Targeted disruption of p70(s6k) defines its role in protein synthesis and rapamycin sensitivity.
Kawasome H, Papst P, Webb S, Keller G M, Johnson G L, Gelfand E W, Terada N
Proc. Natl. Acad. Sci. USA, 95, 5033-5038 (1998). 9560223

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rps6kb1
MGI:1270849		ribosomal protein S6 kinase, polypeptide 111Rps6kb1<tm1Nte> targeted mutation 1, Naohiro Terada
neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal muscle regeneration(MP:0000750)

  • abnormal tibialis anterior morphology(MP:0003083)

  • decreased body size(MP:0001265)

  • decreased epididymal fat pad weight(MP:0009289)

  • decreased inguinal fat pad weight(MP:0009293)
    • more 7 phenotypes
  • decreased interscapular fat pad weight(MP:0009295)

  • decreased mesenteric fat pad weight(MP:0009299)

  • decreased retroperitoneal fat pad weight(MP:0009305)

  • decreased skeletal muscle weight(MP:0010239)

  • extended life span(MP:0001661)

  • increased carbon dioxide production(MP:0008963)

  • increased oxygen consumption(MP:0005289)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Rps6kb1 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Use of the BIOLOGICAL RESOURCE or descendants by for-profit entities for Commercial Purposes requires a use license from DEPOSITOR. RIKEN BRC agrees to inquire whether the party requesting the BIOLOGICAL RESOURCE is a for-profit entity or is using the BIOLOGICAL RESOURCE of descendants for Commercial Purposes, and if so, will advise the party that it must first obtain a use license from DEPOSITOR before receiving the BIOLOGICAL RESOURCE.
    Commercial Purposes shall include, but not be limited to, uses of the BIOLOGICAL RESOURCE of descendants by any organization, including recipient, to perform contract research, to screen compound libraries, to produce or manufacture products for general sale, or to conduct research activities that result in any sale, lease, license, or transfer of industrially sponsored academic research shall not be considered a use of the BIOLOGICAL RESOURCE for Commercial Purposes per se, unless any of the above conditions of this definition are met.
    DepositorErwin W. Gelfand (National Jewish Medical and Research Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Zhao J, Li SW, Gong QQ, Ding LC, Jin YC, Zhang J, Gao JG, Sun XY.
    A disputed evidence on obesity: comparison of the effects of Rcan2(-/-) and Rps6kb1(-/-) mutations on growth and body weight in C57BL/6J mice.
    J Zhejiang Univ Sci B 17(9) 657-71(2016) 27604858