Strain Data Sheet

RBRC02387

Strain Information

Image
BRC No.RBRC02387
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129P2(B6)-Ly86<tm1Kmiy>
Former Common nameBalb/c-MD-1 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainBALB/cCrSlc
Appearance
Strain development
Strain description
Colony maintenance
References
Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide.
Nagai Y, Shimazu R, Ogata H, Akashi S, Sudo K, Yamasaki H, Hayashi S, Iwakura Y, Kimoto M, Miyake K
Blood, 99, 1699-1705 (2002). 11861286

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ly86
MGI:1321404		lymphocyte antigen 8613Ly86<tm1Kmiy>
MGI:3641422		targeted mutation 1, Kensuke Miyake
neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased B cell proliferation (MP:0005093)

  • decreased IgG2b level (MP:0008497)

  • decreased IgG3 level (MP:0008498)

  • increased IgG level (MP:0002493)

  • increased IgM level (MP:0002494)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Ly86 genome DNA, E. coli neo, mouse PGK-1 promoter
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 99, 1699-1705 (2002).
    DepositorKensuke Miyake (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data