Strain Data Sheet

RBRC02388

Strain Information

Image
BRC No.RBRC02388
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ly96<tm1Kmiy>
Former Common nameC57BL/6-MD-2 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Essential role of MD-2 in LPS responsiveness and TLR4 distribution.
Nagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M, Miyake K
Nat. Immunol. 3, 667-672 (2002). 12055629

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ly96
MGI:1341909		lymphocyte antigen 961Ly96<tm1Kmiy>
MGI:3053109		targeted mutation 1, Kensuke Miyake
neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal immune cell physiology(MP:0001819)

  • abnormal macrophage physiology(MP:0002451)

  • decreased B cell proliferation(MP:0005093)

  • decreased interleukin-6 secretion(MP:0008706)

  • decreased susceptibility to endotoxin shock(MP:0008734)
    • more 3 phenotypes
  • decreased tumor necrosis factor secretion(MP:0008561)

  • impaired neutrophil recruitment(MP:0008719)

  • increased susceptibility to bacterial infection(MP:0002412)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ly96 genome DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Immunol. 3, 667-672 (2002).
    DepositorKensuke Miyake (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Wang M, Zhang Q, Lou S, Jin L, Wu G, Wu W, Tang Q, Wang Y, Long X, Huang P, Luo W, Liang G.
    Inhibition of MD2 by natural product-drived JM-9 attenuates renal inflammation and diabetic nephropathy in mice.
    Biomed Pharmacother 168 115660(2023) 37806092
    Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G.
    MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease.
    Clin Transl Med 12(3) e777(2022) 35343085
    Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y.
    Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis.
    Int J Biol Sci 16(8) 1288-1302(2020) 32210720
    Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G.
    MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy.
    Nat Commun 11(1) 2148(2020) 32358497
    Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G.
    Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys.
    Br J Pharmacol 176(14) 2642-2657(2019) 30959575
    Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G.
    Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury.
    Phytomedicine 63 152997(2019) 31254764
    Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y.
    Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
    Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884
    Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G.
    Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease.
    J Cell Mol Med 22(2) 936-947(2018) 29077272
    Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y.
    Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway.
    Toxicol Lett 282 109-120(2018) 29111459
    Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y.
    Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy.
    J Cell Mol Med 21(12) 3776-3786(2017) 28767204
    Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G.
    MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2.
    Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347
    Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G.
    MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis.
    Br J Pharmacol 172(17) 4391-405(2015) 26076332