Strain Data Sheet

RBRC02393

Strain Information

Image
BRC No.RBRC02393
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Aicda<tm1Hon>(N12)
Former Common nameBALB/c-Aicda<-/->(N12)mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Masamichi Muramatsu at Kyoto University in 2000. A neomycin resistance cassette was transferred into TT2 ES cells to replace exon 2 and part of exon 3 of the Aicda gene. The Aid deficient mice were backcrossed to BALB/c.
Strain descriptionAid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency causes a complete defect in class switching and shows a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cells is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene is a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also found that somatic hypermutation of an immunoglobulin variable region gene and gene conversion did not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.
Colony maintenanceHomozyogte x HomozygoteHomozygous mutant mice are viable and fertile.
References
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Muramatsu M, Kinoshita K, Fagarasan S, Yamada S, Shinkai Y, Honjo T
Cell, 102, 553-563 (2000). 11007474

Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora.
Fagarasan S, Muramatsu M, Suzuki K, Nagaoka H, Hiai H, Honjo T
Science, 298, 1424-1427 (2002). 12434060

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:ASentinel mouse program
2023/08/28Room:4-BRack:ASentinel mouse program
2023/05/29Room:4-BRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicda
MGI:1342279		activation-induced cytidine deaminase6Aicda<tm1Hon>
MGI:2156156		targeted mutation 1, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal class switch recombination(MP:0004816)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • chromosomal instability(MP:0008866)

  • decreased IgA level(MP:0001807)

  • decreased IgG level(MP:0001805)
    • more 8 phenotypes
  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • immune system phenotype(MP:0005387)

  • increased IgM level(MP:0002494)

  • increased germinal center B cell number(MP:0008177)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA [Update June, 4, 2015]
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data