Strain Data Sheet

RBRC02396

Strain Information

Image
BRC No.RBRC02396
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Pknox1<tm1.1Nuk>
Former Common namePrep1LacZKIdeltaNeo, C57BL/6
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Atsushi Kuroiwa, Nagoya University.
Strain descriptionPrep1 (Pknox1) knockout mice. Prep1 gene is a member of the TALE class of homeodomain proteins and essential in embryonic development. Homozygous mutant mice are embryonic lethal (E9.5). This strain is useful for elucidation of the function of Prep1 gene during embryonic development.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pknox1
MGI:1201409		Pbx/knotted 1 homeobox17Pknox1<tm1.1Nuk> targeted mutation 1.1, Nagoya University Atsushi Kuroiwa
lacZ beta-galactosidase (E. coli)17
loxP phage P1 loxP17loxP
poly A SV40 polyA17poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality(MP:0008762)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP, E. coli LacZ, SV40 polyA signal sequence, mouse Pknox1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAtsushi Kuroiwa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data