Strain Data Sheet

RBRC02398

Strain Information

Image
BRC No.RBRC02398
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Pknox2<tm1.1Nuk>
Former Common namePrep2LacZKIdeltaNeo, C57BL/6
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Atsushi Kuroiwa, Graduate School of Science, Nagoya University. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette. PGK-neo was excised by crossing with mice expressing cre recombinase.
Strain descriptionPrep2(Pknox2) is a transcriptional regulator having TALE momeodomain, and is involved in transcriptinal regulation by binding to the target sequence as a heterodimer with transcription factor of Pbx having TALE homeodomain. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette. PGK-neo was excised by crossing with mice expressing cre recombinase.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pknox2
MGI:2445415		Pbx/knotted 1 homeobox 29Pknox2<tm1.1Nuk> targeted mutation 1.1, Nagoya University Atsushi Kuroiwa
lacZ beta-galactosidase (E. coli)9
loxP phage P1 loxP9loxP
poly A SV40 polyA9poly A

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased body size (MP:0001265)

  • postnatal growth retardation (MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, E. coli LacZ, SV40 polyA additional signal sequence, mouse Pknox2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAtsushi Kuroiwa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    No Data