Strain Data Sheet

RBRC02399

Strain Information

Image
BRC No.RBRC02399
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-PRAT4A<tm1Kmiy>
Former Common nameC57BL/6-PRAT4A<-/->
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kensuke Miyake, The University of Tokyo in 2000.
Strain descriptionC57BL/6-PRAT4A<tm1Kmiy>. Immune cells such as dendric cells (DCs) and macrophages express multiple Toll-like receptors (TLRs) which recognize a variety of pathogen products. A protein associated with TLR4 (PRAT4A) has been found to bind TLR4, and regulate the expression of TLR4 and the response to LPS. Using PRAT4A-deficient mice, there are defective cytokine production against bacteria and activation of Th1 responses. DCs, macrophages and B cells lacking PRAT4A have impaired responses to a variety of TLR ligands. The PRAT4A-deficient bone marrow chimera mice are resistant to lethal-dose LPS administration in the LPS-induced sepsis model to analyze in vivo immune response. This mutant mouse is useful for the study of PRAT4A in innate and adaptive immunity.
Colony maintenanceHeterozygote x Wild-type [C57BL/6CrSlc]
References
A protein associated with Toll-like receptor (TLR) 4 (PRAT4A) is required for TLR-dependent immune responses.
Takahashi K, Shibata T, Akashi-Takamura S, Kiyokawa T, Wakabayashi Y, Tanimura N, Kobayashi T, Matsumoto F, Fukui R, Kouro T, Nagai Y, Takatsu K, Saitoh S, Miyake K
J. Exp. Med., 204, 2963-2976 (2007). 17998391

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cnpy3
MGI:1919279		canopy FGF signaling regulator 317Cnpy3<tm1Kmiy>
MGI:3767622		targeted mutation 1, Kensuke Miyake
  • developmental and epileptic encephalopathy, 60(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal lymphocyte physiology(MP:0003945)

  • abnormal macrophage physiology(MP:0002451)

  • decreased body size(MP:0001265)

  • decreased interleukin-6 secretion(MP:0008706)

  • decreased tumor necrosis factor secretion(MP:0008561)
    • more 2 phenotypes
  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PGK promoter, E. coli neo, mouse Ptcra genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 204, 2963-2976 (2007). Transfer of the BIOLOGICAL RESOURCE to the third party from the RECIPIENT will never be done without a prior permission of the DEPOSITOR.
    The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE.
    The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKensuke Miyake (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Apr 2008

    BRC mice in Publications

    No Data