Strain Data Sheet

RBRC02400

Strain Information

Image
BRC No.RBRC02400
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129-Tekt2<Gt(Betageo)1Htan>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
References
Mice deficient in the axonemal protein Tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function.
Tanaka H, Iguchi N, Toyama Y, Kitamura K, Takahashi T, Kaseda K, Maekawa M, Nishimune Y
Mol. Cell. Biol., 24, 7958-7964 (2004). 15340058

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
3'-LTR retrovirus LTR (viral long terminal repeat)43'-LTR
5'-LTR retrovirus LTR (viral long terminal repeat)45'-LTR
SA Splice acceptor4SA
Splice donor Splice donor4Splice donor
Tekt2
MGI:1346335		tektin 24Tekt2<Gt(Betageo)1Htan> gene trap insertion 1, Hiromitsu Tanaka
lacZ beta-galactosidase (E. coli)4
neo neomycin resistance gene (E. coli)4
puro puromycin acyltransferase (Streptomyces alboniger)4puro mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mucociliary clearance(MP:0001947)

  • abnormal respiratory motile cilium morphology(MP:0011050)

  • abnormal respiratory motile cilium physiology(MP:0011055)

  • abnormal sperm axoneme morphology(MP:0009838)

  • abnormal tracheal ciliated epithelium morphology(MP:0002285)
    • more 3 phenotypes
  • asthenozoospermia(MP:0002675)

  • hairpin sperm flagellum(MP:0009243)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAThe gene trap vector contained a retroviral 5'-end long terminal (LTR), splice acceptor (SA), E. coli lacZ/neomycin phosphotransferase fusion gene(BetaGeo), pA, mouse phosphoglycerate kinase (PGK)-1 gene promoter, Streptomyces alboniger puromycin N-acetyltransfetase (pac) gene, splice donor (SD) sequence, 3'-LTR
    Research applicationReproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If interested in obtaining materials from the tektin-t knockout mouse line, please contact the Texas Institute for Genomic Medicine directly at www.tigm.org.
    DepositorHiromitsu Tanaka (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data