Strain Data Sheet

RBRC02405

Strain Information

Image
BRC No.RBRC02405
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-4930583H14Rik<tm1Hmd>
Former Common name4930583H14Rik flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
4930583H14Rik
MGI:1914999		RIKEN cDNA 4930583H14 gene34930583H14Rik<tm1Hmd> targeted mutation 1, Hiroshi Hamada
Frt yeast FRT (flippase recombination target) site3Frt
IRES internal ribosomal entry site (EMCV)3
lacZ beta-galactosidase (E. coli)3
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
myc-tag myc-epitope tag3myc-tag
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, epitope tag(mycTAG), yeast FRT (flipase recombination target) site, Phage P1 Cre recombination target sites(loxp), E. coli neo
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data