Strain Data Sheet

RBRC02421

Strain Information

Image
BRC No.RBRC02421
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Bcl11b<tm1Jpk>
Former Common nameBcl11b/Rit1-KO mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Ryo Kominami, Graduate School of Medical and Dental Science, Niigata University. TT2 ES cells derived from B6CBAF1 were used for the generation of knockout mice. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Strain descriptionBcl11b gene knock-out mice. A PGK-neo cassette was inserted into exon 1 of the Bcl11b gene. Bcl11b is a tumor-suppressor gene involved in T cell lymphomas. Homozygous mutant mice diw within the first day after birth. Mutant mice show a block at the CD4-CD8 double-negative stage of thymocyte development without any impairment in cells of B- or gammadelta T cell lineages. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Colony maintenanceHeterozygote x Wild-type [BALB/cAJcl]
References
Bcl11b is required for differentiation and survival of alphabeta T lymphocytes.
Wakabayashi Y, Watanabe H, Inoue J, Takeda N, Sakata J, Mishima Y, Hitomi J, Yamamoto T, Utsuyama M, Niwa O, Aizawa S, Kominami R
Nat. Immunol., 4, 533-539 (2003) 12717433

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcl11b
MGI:1929913		B cell leukemia/lymphoma 11B12Bcl11b<tm1Jpk>
MGI:2663971		targeted mutation 1, Japan Kominami
  • immunodeficiency 49(MedGEN)

  • intellectual developmental disorder with speech delay, dysmorphic facies, and t-cell abnormalities(MedGEN)
    • neo neomycin resistance gene (E. coli)12 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell differentiation(MP:0002145)

  • abnormal T cell morphology(MP:0008037)

  • abnormal T cell receptor beta chain V(D)J recombination(MP:0008758)

  • abnormal ameloblast morphology(MP:0002650)

  • abnormal basicranium morphology(MP:0010029)
    • more 20 phenotypes
  • abnormal incisor morphology(MP:0005358)

  • abnormal thymus cortex morphology(MP:0002371)

  • abnormal thymus corticomedullary boundary morphology(MP:0009543)

  • abnormal thymus medulla morphology(MP:0002375)

  • abnormal tooth development(MP:0000116)

  • absent gastric milk in neonates(MP:0009546)

  • absent thymus corticomedullary boundary(MP:0013741)

  • decreased birth body size(MP:0009703)

  • decreased birth weight(MP:0009674)

  • decreased double-positive T cell number(MP:0005092)

  • eyelids open at birth(MP:0001302)

  • increased CD8-positive, alpha-beta T cell number(MP:0008078)

  • increased double-negative T cell number(MP:0005090)

  • increased thymocyte apoptosis(MP:0009541)

  • lethality at weaning, complete penetrance(MP:0011083)

  • long incisors(MP:0004831)

  • midface hypoplasia(MP:0012085)

  • neonatal lethality, complete penetrance(MP:0011087)

  • short maxilla(MP:0000097)

  • thymus hypoplasia(MP:0001823)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Bcl11b genome DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Immunol., 4, 533-539 (2003). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRyo Kominami (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data