Strain Data Sheet

RBRC02435

Strain Information

Image
BRC No.RBRC02435
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Srpx<tm1Hzi>
Former Common nameDrs KO, Srpx KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yukihiro Tambe and Hirokazu Inoue, Shiga University of Medical Sciences in 2007.
Strain descriptionThe Drs gene is originally found as a novel suppressor of v-src transformation. The Drs gene expresses in most normal tissues, however the expression is completely downregulated in a variety of cancer cell lines and tissues. It has been shown that malignant tumors such as lymphomas, lung adenocarcinomas and hepatomas were generated in about 30% of the Drs KO mice between 7 and 12 months. The Drs KO embryonic fibroblasts also showed enhanced sensitivity to transformation by viral oncogenes. Reintroduction of Drs gene into a tumor cell line derived from the tumor of the Drs KO mouse suppresses tumorigenesis and enhances caspase-mediated apoptosis.
Colony maintenanceHeterozygote (female), Hemizygote (male) x C57BL/6 [C57BL/6JJcl]
References
Tumor prone phenotype of mice deficient in a novel apoptosis-inducing gene, drs.
Tambe Y, Yoshioka-Yamashita A, Mukaisho K, Haraguchi S, Chano T, Isono T, Kawai T, Suzuki Y, Kushima R, Hattori T, Goto M, Yamada S, Kiso M, Saga Y, Inoue H
Carcinogenesis, 28, 777-784 (2007). 17088259

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Srpx
MGI:1858306		sushi-repeat-containing proteinXSrpx<tm1Hzi>
MGI:3706175		targeted mutation 1, Hirokazu Inoue
loxP phage P1 loxPXloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal lymphocyte morphology(MP:0002619)

  • enlarged spleen(MP:0000691)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased hepatoma incidence(MP:0010264)

  • increased lung adenocarcinoma incidence(MP:0002027)
    • more 3 phenotypes
  • increased lymphoma incidence(MP:0012431)

  • increased sarcoma incidence(MP:0002032)

  • increased tumor incidence(MP:0002020)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse srpx genomic DNA, Phage P1 loxP
    Research applicationApoptosis Research
    Cancer Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Carcinogenesis, 28(4), 777-784 (2007).The RECIPENT uses the BIOLOGICAL RESOURCE as a collaborative project with the DEPOSITOR.
    DepositorHirokazu Inoue (Shiga University of Medical Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Jun 2008

    BRC mice in Publications

    No Data