Strain Data Sheet

RBRC02443

Strain Information

Image
BRC No.RBRC02443
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Akr1c18<tm1Mni>
Former Common name20alpha-HSD knock-out mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Reproductive phenotypes in mice with targeted disruption of the 20alpha-hydroxysteroid dehydrogenase gene.
Ishida M, Choi J H, Hirabayashi K, Matsuwaki T, Suzuki M, Yamanouchi K, Horai R, Sudo K, Iwakura Y, Nishihara M
J. Reprod. Dev., 53, 499-508 (2007) 17272929

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Akr1c18
MGI:2145420		aldo-keto reductase family 1, member C1813Akr1c18<tm1Mni>
MGI:3774264		targeted mutation 1, Masugi Nishihara
GFP Green Fluorescent Protein (Aequorea victoria)13
IRES internal ribosomal entry site (EMCV)13
neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased litter size(MP:0001935)

  • increased circulating progesterone level(MP:0005186)

  • long gestation period(MP:0002293)

  • prolonged diestrus(MP:0009011)

  • prolonged estrous cycle(MP:0009006)
    • Detailed phenotype data

    Ordering Information

    Donor DNAマウス20α-水酸化ステロイド脱水素酵素遺伝子ゲノム断片、IRES、EGFP、ネオマイシン耐性遺伝子
    Research applicationReproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev., 53, 499-508 (2007).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorMasugi　Nishihara (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data