Strain Data Sheet

RBRC02463

Strain Information

Image
BRC No.RBRC02463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nefh<tm1(Venus)Ito>
Former Common nameNF-H KO mice
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
GH Growth hormone polyA (Bovine)11GH
Nefh
MGI:97309		neurofilament, heavy polypeptide11Nefh<tm1(Venus)Ito> targeted mutation 1 (venus), Shigeyoshi Itohara
  • amyotrophic lateral sclerosis type 1(MedGEN)

  • Charcot-Marie-Tooth disease axonal type 2CC(MedGEN)
    • Venus Yellow Fluorescent Protein (Aequorea victoria)11Venus
    loxP phage P1 loxP11loxP
    myc-tag myc-epitope tag (rat)11myc-tag
    nls SV40 large T antigen nuclear localization signal (NLS)11nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 LoxP site, Jellyfish Venus cDNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), yeast FRT site, rat Myc-epitope, bovine BGH poly A, mouse Nefh genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data