Strain Data Sheet

RBRC02477

Strain Information

Image
BRC No.RBRC02477
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Grin2a<tm1Nak> Grin2b<tm1Stl> Drd2<tm1Mok> Drd3<tm1Mok> Drd4<tm1Mok>
Former Common nameB6-NR2A/NR2B/D2R/D3R/D4R KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionNR2A (Grin2a), NR2B (Grin2b), D2R (Drd2), D3R (Drd3) and D4R (Drd4) genes multiple knockout mice.
Colony maintenance
References
Dopamine D2 receptor plays a critical role in cell proliferation and proopiomelanocortin expression in the pituitary.
Yamaguchi H, Aiba A, Nakamura K, Nakao K, Sakagami H, Goto K, Kondo H, Katsuki M
Genes Cells. 1(2):253-68 (1996). 9140068

Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
Kadotani H, Hirano T, Masugi M, Nakamura K, Nakao K, Katsuki M, Nakanishi S
J. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drd3
MGI:94925		dopamine receptor D316Drd3<tm1Mok>
MGI:6376625		targeted mutation 1, Motoya Katsuki
  • schizophrenia(MedGEN)

  • tremor, hereditary essential, 1(MedGEN)
    • Ecogpt E.coli xanthine phosphoribosyltransferase16Ecogpt mouse phosphoglycerate kinase promoter (PGK promoter)
    Grin2a
    MGI:95820    		glutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>
    MGI:1928506    		targeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
    • neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Grin2b
    MGI:95821    		glutamate receptor, ionotropic, NMDA2B (epsilon 2)6Grin2b<tm1Stl> targeted mutation 1, Susumu Tonegawa
  • developmental and epileptic encephalopathy, 27(MedGEN)

  • intellectual disability, autosomal dominant 6(MedGEN)
    • neo neomycin resistance gene (E. coli)6
    Drd4
    MGI:94926    		dopamine receptor D47Drd4<tm1Mok>
    MGI:6376626    		targeted mutation 1, Motoya Katsuki
  • attention deficit-hyperactivity disorder(MedGEN)
    • Ecogpt E.coli xanthine phosphoribosyltransferase7Ecogpt mouse phosphoglycerate kinase promoter (PGK promoter)
    neo neomycin resistance gene (E. coli)7 mouse RNA polymerase II large subunit promoter
    Drd2
    MGI:94924    		dopamine receptor D29Drd2<tm1Mok>
    MGI:3576789    		targeted mutation 1, Motoya Katsuki
  • cocaine dependence(DisGeNET)
    • neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal circulating hormone level (MP:0005418)

  • abnormal physiological response to xenobiotic (MP:0008872)

  • abnormal single cell response (MP:0003463)

  • behavior/neurological phenotype (MP:0005386)

  • darkened coat color (MP:0005409)
    • more 22 phenotypes
  • decreased exploration in new environment (MP:0001417)

  • decreased grooming behavior (MP:0001442)

  • decreased locomotor activity (MP:0001402)

  • decreased systemic arterial systolic blood pressure (MP:0006264)

  • decreased urine flow rate (MP:0011432)

  • decreased urine sodium level (MP:0006317)

  • enhanced behavioral response to cocaine (MP:0009754)

  • enhanced coordination (MP:0003858)

  • enlarged pituitary intermediate lobe (MP:0008370)

  • hyperactivity (MP:0001399)

  • hyperactivity elicited by ethanol administration (MP:0002629)

  • impaired behavioral response to addictive substance (MP:0009750)

  • impaired behavioral response to morphine (MP:0009757)

  • impaired conditioned place preference behavior (MP:0009712)

  • increased dopamine level (MP:0001906)

  • increased exploration in new environment (MP:0001415)

  • increased pituitary hormone level (MP:0003973)

  • increased renin activity (MP:0005582)

  • increased systemic arterial diastolic blood pressure (MP:0006143)

  • increased systemic arterial systolic blood pressure (MP:0006144)

  • increased vertical activity (MP:0002574)

  • nervous system phenotype (MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli xanthine phosphoribosyltransferase(Ecogpt), mouse RNA polymerase II large subunit promoter, mouse NMDAR2A genomic DNA, mouse NMDA2B genomic DNA, mouse Drd2 genomic DNA, mouse Drd3 genomic DNA, mouse Drd4 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Nerurosci., 16, 7859-7867 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Drs. Motoya Katsuki and Toshikuni Sasaoka is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data