Strain Data Sheet

RBRC02479

Strain Information

Image
BRC No.RBRC02479
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Hras1<tm1Mok> Grin2a<tm1Nak> Drd1a<tm1Mok> Drd5<tm1Mok>
Former Common nameB6-Hras/NR2A/D1R/D5R KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionHras, NR2A (Grin2a), D1R (Drd1a) and D5R (Drd5) genes multiple knockout mice.
Colony maintenance
References
Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
Kadotani H, Hirano T, Masugi M, Nakamura K, Nakao K, Katsuki M, Nakanishi S
J. Neurosci., 16, 7859-7867 (1996). 8987814

Targeted deletion of the H-ras gene decreases tumor formation in mouse skin carcinogenesis.
Ise K, Nakamura K, Nakao K, Shimizu S, Harada H, Ichise T, Miyoshi J, Gondo Y, Ishikawa T, Aiba A, Katsuki M
Oncogene, 19, 2951-2956 (2000). 10871846

Dopamine D1 receptor modulates hippocampal representation plasticity to spatial novelty.
Tran A H, Uwano T, Kimura T, Hori E, Katsuki M, Nishijo H, Ono T
J. Neurosci., 28(50):13390-13400 (2008). 19074012

Methamphetamine increases locomotion and dopamine transporter activity in dopamine d5 receptor-deficient mice.
Hayashizaki S, Hirai S, Ito Y, Honda Y, Arime Y, Sora I, Okado H, Kodama T, Takada M
PLoS One, 8(10):e75975 (2013). 24155877

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drd1
MGI:99578
dopamine receptor D113Drd1<tm1Mok>
MGI:3829942
targeted mutation 1, Motoya Katsuki
Ecogpt E.coli xanthine phosphoribosyltransferase13Ecogpt mouse phosphoglycerate kinase promoter (PGK promoter)
neo neomycin resistance gene (E. coli)13 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Grin2a
MGI:95820
glutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>
MGI:1928506
targeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
  • neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Drd5
    MGI:94927
    dopamine receptor D55Drd5<tm1Mok>
    MGI:5569103
    targeted mutation 1, Motoya Katsuki
  • attention deficit-hyperactivity disorder(MedGEN)

  • benign essential blepharospasm(MedGEN)
  • Ecogpt E.coli xanthine phosphoribosyltransferase5Ecogpt mouse phosphoglycerate kinase promoter (PGK promoter)
    neo neomycin resistance gene (E. coli)5 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Hras
    MGI:96224
    Harvey rat sarcoma virus oncogene7Hras<tm1Mok>
    MGI:2386128
    targeted mutation 1, Motoya Katsuki
  • Costello syndrome(DisGeNET, MedGEN)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)
  • more 4 Diseases
  • melanoma(DisGeNET)

  • nevus, epidermal(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)

  • urinary bladder cancer(MedGEN)
  • neo neomycin resistance gene (E. coli)7

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal adrenal gland physiology (MP:0002909)

  • abnormal excitatory postsynaptic currents (MP:0002910)

  • abnormal kidney physiology (MP:0002136)

  • abnormal miniature excitatory postsynaptic currents (MP:0004753)

  • abnormal sympathetic nervous system physiology (MP:0004183)
  • more 11 phenotypes
  • abnormal synaptic plasticity (MP:0004859)

  • behavior/neurological phenotype (MP:0005386)

  • chylous ascites (MP:0012080)

  • decreased incidence of tumors by chemical induction (MP:0004502)

  • decreased vertical activity (MP:0002757)

  • hypertension (MP:0000231)

  • increased heart weight (MP:0002833)

  • increased systemic arterial blood pressure (MP:0002842)

  • increased systemic arterial diastolic blood pressure (MP:0006143)

  • increased systemic arterial systolic blood pressure (MP:0006144)

  • nervous system phenotype (MP:0003631)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse NMDAR2A genomic DNA, mouse Hras genomic DNA, mouse Drd1a genomic DNA, mouse Drd5 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli xanthine phosphoribosyltransferase(Ecogpt)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 19, 2951-2956 (2000). J. Neurosci., 16, 7859-7867 (1996). J. Neurosci., 28, 13390-13400 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data