Strain Data Sheet

RBRC02492

Strain Information

Image
BRC No.RBRC02492
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1/Mesp2<tm1Ysa>Tg(deltaP2deltaEME)2Ysa/YsaRbrc
Former Common nameBAC- Delta P2 Delta EME#2/Mesp1 Mesp2-dko
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology.
Oginuma M, Hirata T, Saga Y
Mech. Dev., 125, 432-440 (2008) 18328678

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp1/Mesp2 mesoderm posterior 17Mesp1/Mesp2<tm1Ysa>
MGI:2386200		targeted mutation 1, Yumiko Saga
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)
ALP alkaline phosphatase (human)UNALP
Frt yeast FRT (flippase recombination target) siteUNFrt

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal somite development(MP:0001688)

  • abnormal vertebral arch morphology(MP:0004599)

  • abnormal vertebral lamina morphology(MP:0004605)

  • abnormal vertebral pedicle morphology(MP:0004604)

  • abnormal vertebral transverse process morphology(MP:0004600)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman alkaline phosphatase-pA cDNA, yeast Frt, BAC(Bacterial artificial chromosome)clone containing mouse Mesp1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech Dev., 125, 432-440 (2008).
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data