Strain Information | |
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Image | |
BRC No. | RBRC02498 |
Type | Transgene |
Species | Mus musculus |
Strain name | ICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc |
Former Common name | BAC-stra8EGFP-bghpA#1 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | Unfixed |
Strain development | Developed by Dr. Yumiko Saga, National Institute of Genetics in 2007. ICR mixed background. |
Strain description | BAC transgenic mice having a fusion gene of Stra8 and EGFP, which also contain a beta-globin polyA signal. |
Colony maintenance | Carrier x NoncarrierTg mice are viable and fertile.According to a breeding result, the transgene may be integrated into chromosome Y. |
References | Biol Reprod., 89(1):3 (2013). 23677978 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2beta-globin polyA signalUNHBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)UN |
Ordering Information | |
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Donor DNA | Jellyfish EGFP cDNA, beta-globin-polyA signal, mouse Stra8 genomic DNA, yeast FRT (flipase recombination target) sites, E. coli neo, DNA from BAC clone RP23-367A8 |
Research application | Developmental Biology Research FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors. |
Depositor | Yumiko Saga (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y. Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes. Biol Reprod 89(1) 3(2013) 23677978 |