Strain Data Sheet

RBRC02498

Strain Information

Image
BRC No.RBRC02498
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc
Former Common nameBAC-stra8EGFP-bghpA#1
H-2 Haplotype
ES Cell line
Background strain
AppearanceUnfixed
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2007. ICR mixed background.
Strain descriptionBAC transgenic mice having a fusion gene of Stra8 and EGFP, which also contain a beta-globin polyA signal.
Colony maintenanceCarrier x NoncarrierTg mice are viable and fertile.According to a breeding result, the transgene may be integrated into chromosome Y.
References
Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y
Biol Reprod., 89(1):3 (2013). 23677978

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
Frt yeast FRT (flippase recombination target) siteUNFrt
GFP Green Fluorescent Protein (Aequorea victoria)UN
HBB2 beta-globin polyA signalUNHBB2
Stra8
MGI:107917		stimulated by retinoic acid gene 8UNStra8
neo neomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish EGFP cDNA, beta-globin-polyA signal, mouse Stra8 genomic DNA, yeast FRT (flipase recombination target) sites, E. coli neo, DNA from BAC clone RP23-367A8
    Research applicationDevelopmental Biology Research
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y.
    Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
    Biol Reprod 89(1) 3(2013) 23677978