Strain Data Sheet

RBRC02508

Strain Information

Image
BRC No.RBRC02508
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameΔneo-mNos2-L(N2enh-nos2(dZ)EGFP)#2
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2007.
Strain descriptionThis mutant mouse expreses a Nanos2-EGFP fusion protein lacking a C terminal region with a zinc finger motif in a Nanos2-expressing cell on Nanos2 knockout background.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN Nanos2 enhancer promoter
Nanos2 nanos homolog 2 (Drosophila)UNNanos2 Nanos2 enhancer promoter
lacZ beta-galactosidase (E. coli)UN
loxP phage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNANanos2 enhancer promoter, Nanos2 (ΔZ)EGFP LacZ 遺伝子, lox 配列
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data