Strain Data Sheet

RBRC02565

Strain Information

Image
BRC No.RBRC02565
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc
Former Common nameNanos3-CrepA(neo)#35
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga (2007).
Strain descriptionThese knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile.
Colony maintenanceSibling Mating (Hemizygote x Wild-type)
References
Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
Suzuki H, Tsuda M, Kiso M, Saga Y
Dev. Biol., 318, 133-142 (2008). 18436203

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site8Frt
Frt yeast FRT (flippase recombination target) site8Frt
Nanos3
MGI:2675387		nanos C2HC-type zinc finger 38Nanos3<tm2(cre)Ysa>
MGI:3777592		targeted mutation 2 (cre), Yumiko Saga
cre Phage P1 Cre recombinase8cre
neo neomycin resistance gene (E. coli)8 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal female germ cell morphology(MP:0006361)

  • abnormal female meiosis(MP:0005168)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse nanos3 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 318, 133-142 (2008).Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Ogonuki N, Kyogoku H, Hino T, Osawa Y, Fujiwara Y, Inoue K, Kunieda T, Mizuno S, Tateno H, Sugiyama F, Kitajima TS, Ogura A.
    Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes.
    EMBO Rep 23(7) e54992(2022) 35587095