Strain Data Sheet

RBRC02566

Strain Information

Image
BRC No.RBRC02566
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos3<tm3(lacZ)Ysa>/YsaRbrc
Former Common nameNanos3-LpA(neo)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Saga Y., 2008. Mouse nanos proteins are essential for germ cell development. In Genetics and Epigenetic Control of Mammalian Germ Cell Development and Function (Nozaki M. edited.) Research Singpost, Kerala, India, 2008.

The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
Suzuki H, Sada A, Yoshida S, Saga Y
Dev Biol., 336(2):222-31 (2009). 19818747

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nanos3
MGI:2675387		nanos C2HC-type zinc finger 38Nanos3<tm3(lacZ)Ysa> targeted mutation 3 (lacZ), Yumiko Saga
lacZ beta-galactosidase (E. coli)8
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP
neo neomycin resistance gene (E. coli)8 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • female infertility(MP:0001926)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, SV40 pA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nanos3 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Saga Y., 2008. Mouse nanos proteins are essential for germ cell development. In Genetics and Epigenetic Control of Mammalian Germ Cell Development and Function (Nozaki M. edited.) Research Singpost, Kerala, India, 2008.Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data