Strain Data Sheet

RBRC02590

Strain Information

Image
BRC No.RBRC02590
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Jam2<tm1Okud>/SiaRbrc
Former Common nameJam-B(IRES-βgeo) (Acc. No. CDB0413K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Putative "stemness" gene jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells.
Sakaguchi T, Nishimoto M, Miyagi S, Iwama A, Morita Y, Iwamori N, Nakauchi H, Kiyonari H, Muramatsu M, Okuda A
Mol. Cell Biol., 26, 6557-6570 (2006). 16914739

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2
IRES internal ribosomal entry site (EMCV)16
Jam2
MGI:1933820		junction adhesion molecule 216Jam2<tm1Okud>
MGI:3663271		targeted mutation 1, Akihiko Okuda
  • basal ganglia calcification, idiopathic, 8, autosomal recessive(MedGEN)
    • lacZ beta-galactosidase (E. coli)16
    neo neomycin resistance gene (E. coli)16

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse En-2 intron (splicing acceptor), encephalomyocarditis virus internal ribosomal entry site, E. coli beta-galactosidase, E. coli neomycin resistance gene, (beta-geo), mouse PGK gene polyadenylation signal, mouse Jam2 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Akihiko OKUDA (akiokuda@saitama-med.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0413K and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Mol Cell Biol. 2006 Sep; 26 (17): 6557-70. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data