Strain Data Sheet

RBRC02591

Strain Information

Image
BRC No.RBRC02591
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Git2<tm1Hsb>/SiaRbrc
Former Common nameGIT2(-) (Acc. No. CDB0414K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Neutrophil direction sensing and superoxide production linked by the GTPase-activating protein GIT2.
Mazaki Y, Hashimoto S, Tsujimura T, Morishige M, Hashimoto A, Aritake K, Yamada A, Nam J M, Kiyonari H, Nakao K, Sabe H
Nat. Immunol., 7, 724-731 (2006). 16715100

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Git2
MGI:1347053
GIT ArfGAP 25Git2<tm1Hsb>
MGI:3664625
targeted mutation 1, Hisataka Sabe
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal lung compliance(MP:0002333)

  • abnormal neutrophil differentiation(MP:0002415)

  • abnormal neutrophil physiology(MP:0002463)

  • enlarged spleen(MP:0000691)

  • extramedullary hematopoiesis(MP:0000240)
  • more 3 phenotypes
  • impaired neutrophil chemotaxis(MP:0008720)

  • increased susceptibility to fungal infection(MP:0005399)

  • lung inflammation(MP:0001861)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neo, Phage P1 loxP、 mouse PKG promoter, mouse PKG 3’ end
    Research applicationImmunology and Inflammation Research
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Hisataka SABE (sabe@obi.or.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0414K and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Nat Immunol. 2006 Jul; 7 (7): 724-31. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Le H, Wang Y, Zhou J, Li D, Gong Z, Zhu F, Wang J, Tian C, Cai W, Wu J.
    Git2 deficiency promotes MDSCs recruitment in intestine via NF-κB-CXCL1/CXCL12 pathway and ameliorates necrotizing enterocolitis.
    Mucosal Immunol (2024) 39074614
    Hao YE, He DF, Yin RH, Chen H, Wang J, Wang SX, Zhan YQ, Ge CH, Li CY, Yu M, Yang XM.
    GIT2 deficiency attenuates concanavalin A-induced hepatitis in mice.
    FEBS Open Bio 5 688-704(2015) 26380813
    Wei J, Wei C, Wang M, Qiu X, Li Y, Yuan Y, Jin C, Leng L, Wang J, Yang X, He F.
    The GTPase-activating protein GIT2 protects against colitis by negatively regulating Toll-like receptor signaling.
    Proc Natl Acad Sci U S A 111(24) 8883-8(2014) 24879442