Strain Data Sheet

RBRC02594

Strain Information

Image
BRC No.RBRC02594
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sept3<tm1Keng>/SiaRbrc
Former Common nameSept3(-) (Acc. No. CDB0422K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Mineko Kengaku, RIKEN Brain Science Institute. A neomycin resistance cassette flanked by loxP sites was transferred into TT2 ES cells to replace a partial region of exon 2 and exons 3-5 of Sept3 gene.
Strain descriptionSept3 knock-out mice. Septins are a family of GTPase proteins that are required for cytokinesis in most eukaryotes. Sept3 is a brain-specific protein that is abundant in synaptic terminals. Sept3 knockout mutant mice show normal histogenesis and neuronal differentiation.
Colony maintenance
References
Targeted disruption of Sept3, a heteromeric assembly partner of Sept5 and Sept7 in axons, has no effect on developing CNS neurons.
Fujishima K, Kiyonari H, Kurisu J, Hirano T, Kengaku M
J. Neurochem., 102, 77-92 (2007). 17564677

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sept3
MGI:1345148		septin 315Sept3<tm1Keng>
MGI:3715203		targeted mutation 1, Mineko Kengaku
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP
neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sept3 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Mineko KENGAKU (kengaku@brain.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0422K and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, J Neurochem. 2007 Jul; 102 (1): 77-92. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data