Strain Data Sheet

RBRC02599

Strain Information

Image
BRC No.RBRC02599
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameIVCS.129P2(B6)-Akr1c18<tm1Mni>
Former Common name20a-HSD IVCS
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainIVCS
Appearance
Strain development
Strain description
Colony maintenance
References
Reproductive phenotypes in mice with targeted disruption of the 20alpha-hydroxysteroid dehydrogenase gene.
Ishida M, Choi J H, Hirabayashi K, Matsuwaki T, Suzuki M, Yamanouchi K, Horai R, Sudo K, Iwakura Y, Nishihara M
J. Reprod. Dev., 53, 499-508 (2007) 17272929

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Akr1c18
MGI:2145420		aldo-keto reductase family 1, member C1813Akr1c18<tm1Mni>
MGI:3774264		targeted mutation 1, Masugi Nishihara
GFP Green Fluorescent Protein (Aequorea victoria)13
IRES internal ribosomal entry site (EMCV)13
neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased litter size(MP:0001935)

  • increased circulating progesterone level(MP:0005186)

  • long gestation period(MP:0002293)

  • prolonged diestrus(MP:0009011)

  • prolonged estrous cycle(MP:0009006)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish EGFP cDNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine Growth Hormone poly-A signal,mouse 20 alpha-hydroxysteroid dehydrogenase genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Reproductive Biology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorMasugi　Nishihara (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data