Strain Data Sheet

RBRC02645

Strain Information

Image
BRC No.RBRC02645
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Mybl2<tm2Sis>
Former Common nameB-Myb<flox/+>, B6.Cg-B-Myb<tm2Sis>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shunsuke Ishii, RIKEN Tsukuba Institute in 2008. A loxP site and a floxed neo cassette were inserted upstream and downstream of exon 5, respectively. The neo cassette was removed by transient cre expression in ES cells.
Strain descriptionB-Myb floxed mice.
Colony maintenance
References
A B-Myb complex containing clathrin and filamin is required for mitotic spindle function.
Yamauchi T, Ishidao T, Nomura T, Shinagawa T, Tanaka Y, Yonemura S, Ishii S
EMBO J., 27, 1852-1862 (2008). 18548008

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mybl2
MGI:101785		myeloblastosis oncogene-like 22Mybl2<tm2Sis>
MGI:3801680		targeted mutation 2, Shunsuke Ishii
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse Mybl2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 27, 1852-1862 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShunsuke Ishii (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data