Strain Data Sheet

RBRC02653

Strain Information

Image
BRC No.RBRC02653
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Tsix/Xist<tm3Sado>
Former Common nameTsix<pA>; B6.Cg-Tsix/Xist<tm3Sado>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2006. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain descriptionTsix<pA> mice. The fragment containing a second intron of the human gamma-globin gene, which harbors an mpA cassette in an antisense orientation with respect to Xist transcription was inserted into exon 1 of Xist. Antisense transcription across the Xist promoter is diminished on this allele. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
References
Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification.
Ohhata T, Hoki Y, Sasaki H, Sado T
Development 135, 227-235 (2008). 18057104

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SA Splice acceptor (human)XSA
Splice donor splicing donar (human)XSplice donor
Tsix
MGI:1336196		X (inactive)-specific transcript, opposite strandXTsix<tm3Sado>
MGI:3772875		targeted mutation 3, Takashi Sado
Xist
MGI:98974		inactive X specific transcriptsXXist<tm3Sado>
MGI:3772875		targeted mutation 3, Takashi Sado
loxP phage P1 loxPXloxP
poly A SV40 multiple polyA signalsXpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA methylation(MP:0008877)

  • abnormal embryo development(MP:0001672)

  • preweaning lethality, incomplete penetrance(MP:0011110)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Xist genomic DNA, Phage P1 loxP, poly A (multiple poly A signals) SV40, splicing acceptor (human), splicing donar (human)
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorTakashi Sado (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2009

    BRC mice in Publications

    No Data