Strain Information | |
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Image | |
BRC No. | RBRC02655 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;129-Xist<tm5Sado> |
Former Common name | Xist<delta A>; B6.Cg-Xist<tm5Sado> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Takashi Sado, National Institute of Genetics in 2007. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6). |
Strain description | Xist<delta> mice. The portion of exon 1 encoding the A repeat was deleted. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). |
Colony maintenance | Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] |
References | Development, 136 (1) 139-146 (2009). 19036803 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/19Room:4-ARack:D 2023/11/20Room:4-ARack:D 2023/08/21Room:4-ARack:D 2023/05/22Room:4-ARack:D 2023/02/20Room:4-ARack:D 2022/11/21Room:4-ARack:D 2022/08/22Room:4-ARack:D 2022/05/23Room:4-ARack:D |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, mouse Xist genomic DNA |
Research application | Cre/loxP system Developmental Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. |
Depositor | Takashi Sado (National Institute of Genetics) |
Strain Status | Live mice Frozen embryos |
Strain Availability | Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2009 |
BRC mice in Publications |
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Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H. Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice. PLoS Genet 12(10) e1006375(2016) 27788132 |