Strain Data Sheet

RBRC02657

Strain Information

Image
BRC No.RBRC02657
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>
Former Common nameROSA nls LacZ
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionPGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Genetic dissection of medial habenula-interpeduncular nucleus pathway function in mice.
Kobayashi Y, Sano Y, Vannoni E, Goto H, Suzuki H, Oba A, Kawasaki H, Kanba S, Lipp H P, Murphy N P, Wolfer D P, Itohara S
Front. Behav. Neurosci., 7:17 (2013). 23487260

Health Report

Examination Date / Room / Rack2025/01/07Room:3-ARack:KSentinel mouse program
2024/10/07Room:3-ARack:KSentinel mouse program
2024/07/08Room:3-ARack:KSentinel mouse program
2024/04/08Room:3-ARack:KSentinel mouse program
2024/01/09Room:3-ARack:KSentinel mouse program
2023/10/10Room:3-ARack:KSentinel mouse program
2023/07/10Room:3-ARack:KSentinel mouse program
2023/04/10Room:3-ARack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735
gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> targeted mutation 1, Shigeyoshi Itohara
lacZ beta-galactosidase (E. coli)6
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
nls SV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAsynthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17, (2013).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2013

    BRC mice in Publications

    Namiki T, Kamoshita M, Kageyama A, Terakawa J, Ito J, Kashiwazaki N.
    Utility of progesterone receptor-ires-Cre to generate conditional knockout mice for uterine study.
    Anim Sci J 92(1) e13615(2021) 34402137
    Yoshimura Y, Ida-Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, Yagoto M, Kawai K, Takahashi T, Nakayama M, Ito M.
    Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice.
    Transgenic Res 27(2) 193-201(2018) 29546522