Strain Data Sheet
RBRC02657
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC02657 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> |
| Former Common name | ROSA nls LacZ |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J. |
| Strain description | PGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Genetic dissection of medial habenula-interpeduncular nucleus pathway function in mice. Kobayashi Y, Sano Y, Vannoni E, Goto H, Suzuki H, Oba A, Kawasaki H, Kanba S, Lipp H P, Murphy N P, Wolfer D P, Itohara S Front. Behav. Neurosci., 7:17 (2013). 23487260 |
Health Report | |
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| Examination Date / Room / Rack | 2025/01/07Room:3-ARack:KSentinel mouse program 2024/10/07Room:3-ARack:KSentinel mouse program 2024/07/08Room:3-ARack:KSentinel mouse program 2024/04/08Room:3-ARack:KSentinel mouse program 2024/01/09Room:3-ARack:KSentinel mouse program 2023/10/10Room:3-ARack:KSentinel mouse program 2023/07/10Room:3-ARack:KSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> | targeted mutation 1, Shigeyoshi Itohara | |||
| lacZ | beta-galactosidase (E. coli) | 6 | |||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
| nls | SV40 nuclear localaization signal (chemically-synthesized nucleotide) | 6 | nls | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | synthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17, (2013). |
| Depositor | Shigeyoshi Itohara (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2013 |
BRC mice in Publications |
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Namiki T, Kamoshita M, Kageyama A, Terakawa J, Ito J, Kashiwazaki N. Utility of progesterone receptor-ires-Cre to generate conditional knockout mice for uterine study. Anim Sci J 92(1) e13615(2021) 34402137 |
Yoshimura Y, Ida-Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, Yagoto M, Kawai K, Takahashi T, Nakayama M, Ito M. Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res 27(2) 193-201(2018) 29546522 |