Strain Data Sheet

RBRC02673

Strain Information

Image
BRC No.RBRC02673
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Papolb<tm1Tba>
Former Common nameTpap KO, C57BL/6
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Regulation of spermatogenesis by testis-specific, cytoplasmic poly(A) polymerase TPAP.
Kashiwabara S, Noguchi J, Zhuang T, Ohmura K, Honda A, Sugiura S, Miyamoto K, Takahashi S, Inoue K, Ogura A, Baba T
Science, 298, 1999-2002 (2002). 12471261

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Papolb
MGI:1932115		poly (A) polymerase beta (testis specific)5Papolb<tm1Tba>
MGI:2447177		targeted mutation 1, Tadashi Baba
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal male germ cell apoptosis(MP:0008280)

  • arrest of spermatogenesis(MP:0001155)

  • decreased testis weight(MP:0004852)

  • male infertility(MP:0001925)

  • small seminiferous tubules(MP:0001153)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phosphoglycerate kinase-1 promoter, Mouse phosphoglycerate kinase-1 polyadenylation sequence, Escherichia coli neomycin resistance gene, Mouse Papolb/Tpap genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTadashi Baba (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data