Strain Data Sheet

RBRC02674

Strain Information

Image
BRC No.RBRC02674
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Phf21a<tm1Tba>
Former Common nameBhc80 KO, C57BL/6
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A component of BRAF-HDAC complex, BHC80, is required for neonatal survival in mice.
Iwase S, Shono N, Honda A, Nakanishi T, Kashiwabara S, Takahashi S, Baba T
FEBS Lett., 580, 3129-3135 (2006) 16684532

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Phf21a
MGI:2384756		PHD finger protein 21A2Phf21a<tm1Tba>
MGI:3629054		targeted mutation 1, Tadashi Baba
  • intellectual developmental disorder with behavioral abnormalities and craniofacial dysmorphism with or without seizures(MedGEN)
    • neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal suckling behavior(MP:0001436)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHerpes simplex virus thymidine kinase promoter, Herpes simplex virus thymidine kinase polyadenylation sequence, Escherichia coli neomycin resistance gene, Mouse Phf21a/Bhc80 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTadashi Baba (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Porter RS, An S, Gavilan MC, Nagai M, Murata-Nakamura Y, Zhou B, Bonefas KM, Dionne O, Manuel JM, St-Germain J, Gascon S, Kim J, Browning L, Laurent B, Cho US, Iwase S.
    Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.
    Cell Rep 44(1) 115213(2025) 39817906