Strain Data Sheet

RBRC02680

Strain Information

Image
BRC No.RBRC02680
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129S2-Spam1<tm1Tba>
Former Common namePh-20 KO, ICR
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg.
Baba D, Kashiwabara S, Honda A, Yamagata K, Wu Q, Ikawa M, Okabe M, Baba T
J. Biol. Chem., 277, 30310-30314 (2002) 12065596

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spam1
MGI:109335		sperm adhesion molecule 16Spam1<tm1Tba>
MGI:2386404		targeted mutation 1, Tadashi Baba
neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal sperm physiology(MP:0004543)

  • impaired fertilization(MP:0000242)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHerpes simplex virus thymidine kinase promoter, Herpes simplex virus thymidine kinase polyadenylation sequence, Escherichia coli neomycin resistance gene, Mouse Spam1/Ph-20 genomic DNA
    Research applicationReproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTadashi Baba (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data