Strain Data Sheet

RBRC02692

Strain Information

Image
BRC No.RBRC02692
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Lilrb4a<tm1Ttk>
Former Common namegp49B
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Toshiyuki Takai, Tohoku University in 2008.
Strain descriptionLilrb4 knock-out mice. Lilrb4 (gp49B) is a member of the immunoglobulin superfamily, containing immunoreceptor tyrosine-based inhibitory motifs that can deliver an inhibitor signal in mast and NK cells.
Colony maintenanceSibling x Sibling (Homozygote x Homozygote)Homozygous mice are viable and fertile.
References
Association of tyrosine phosphatases SHP-1 and SHP-2, inositol 5-phosphatase SHIP with gp49B1, and chromosomal assignment of the gene.
Kuroiwa A, Yamashita Y, Inui M, Yuasa T, Ono M, Nagabukuro A, Matsuda Y, Takai T
J. Biol. Chem., 273, 1070-1074 (1998). 9422771

Stimulatory function of gp49A, a murine Ig-like receptor, in rat basophilic leukemia cells.
Lee K H, Ono M, Inui M, Yuasa T, Takai T
J. Immunol., 165, 4970-4977 (2000). 11046024

A novel regulatory role of gp49B on dendritic cells in T-cell priming.
Kasai S, Inui M, Nakamura K, Kakizaki Y, Endo S, Nakamura A, Ito S, Takai T
Eur. J. Immunol., 38 (9) 2426-37 (2008). 18792399

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Lilrb4a
MGI:102701		leukocyte immunoglobulin-like receptor, subfamily B, member 4A10Lilrb4a<tm1Ttk>
MGI:3818260		targeted mutation 1, Toshiyuki Takai
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dendritic cell antigen presentation(MP:0002455)

  • increased susceptibility to graft versus host disease(MP:0005672)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Lilrb4a genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 273, 1070-1074 (1998). J. Immunol., 165, 4970-4977 (2000).
    DepositorToshiyuki Takai (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Li Z, Zhao M, Li T, Zheng J, Liu X, Jiang Y, Zhang H, Hu X.
    Decidual Macrophage Functional Polarization during Abnormal Pregnancy due to Toxoplasma gondii: Role for LILRB4.
    Front Immunol 8 1013(2017) 28883820