Strain Data Sheet

RBRC02694

Strain Information

Image
BRC No.RBRC02694
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ptk2<tm1Mmsh>
Former Common namePtk2<flox>, fFak
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M
Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751

Effects of FAK ablation on cerebellar foliation, Bergmann glia positioning and climbing fiber territory on Purkinje cells.
Watanabe F, Miyazaki T, Takeuchi T, Fukaya M, Nomura T, Noguchi S, Mori H, Sakimura K, Watanabe M, Mishina M
Eur. J. Neurosci., 27, 836-854 (2008). 18279360

Health Report

Examination Date / Room / Rack2023/11/27Room:4-CRack:ISentinel mouse program
2023/08/28Room:4-CRack:ISentinel mouse program
2023/05/29Room:4-CRack:ISentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site15Frt
Ptk2
MGI:95481		PTK2 protein tyrosine kinase 215Ptk2<tm1Mmsh>
MGI:3777585		targeted mutation 1, Masayoshi Mishina
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Purkinje cell innervation(MP:0003894)

  • abnormal innervation(MP:0002184)

  • ectopic Bergmann glia cells(MP:0002763)

  • reduced cerebellar foliation(MP:0009719)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ptk2 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. Meurosci., 27, 836-854 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
    DepositorMasayoshi Mishina (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data