Strain Data Sheet

RBRC02698

Strain Information

Image
BRC No.RBRC02698
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rapgef2<tm1.1Tkat>
Former Common nameRA-GEF-1<+/->
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strainC57BL/6 (semicongenic)
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. The mice were crossed with Tg(ACTB-cre)13Miya mice. C57BL/6 semicongenic.
Strain descriptionRA-GEF-1 (Rapgef2) gene knockout mice. Exon 15 was removed. Homozygous knockout mice are embryonic lethal due to an abnormal vascular development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1.
Wei P, Satoh T, Edamatsu H, Aiba A, Setsu T, Terashima T, Kitazawa S, Nakao K, Yoshikawa Y, Tamada M, Kataoka T
Biochem. Biophys. Res. Commun., 363, 106-112 (2007). 17826737

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rapgef2
MGI:2659071		Rap guanine nucleotide exchange factor (GEF) 23Rapgef2<tm1.1Tkat>
MGI:3760243		targeted mutation 1.1, Tohru Kataoka
  • epilepsy, familial adult myoclonic, 7(MedGEN)
    • loxP phage P1 loxP3loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal vascular development(MP:0000259)

  • abnormal vasculogenesis(MP:0001622)

  • abnormal visceral yolk sac blood island morphology(MP:0011204)

  • absent vitelline blood vessels(MP:0001719)

  • anencephaly(MP:0001890)
    • more 11 phenotypes
  • decreased embryo size(MP:0001698)

  • decreased erythrocyte cell number(MP:0002875)

  • delayed embryo turning(MP:0004188)

  • embryonic growth arrest(MP:0001730)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • enlarged allantois(MP:0004556)

  • incomplete embryo turning(MP:0001701)

  • pale yolk sac(MP:0001722)

  • small placenta(MP:0004259)

  • spina bifida(MP:0003054)

  • wavy neural tube(MP:0000930)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Rapgef2 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 363, 106-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTohru Kataoka (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data