Strain Data Sheet

RBRC02699

Strain Information

Image
BRC No.RBRC02699
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rapgef6<tm1.1Tkat>
Former Common nameRA-GEF-2<-/->
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. The mice were crossed with Tg(ACTB-cre)13Miya mice. C57BL/6 semicongenic.
Strain descriptionRA-GEF-2 (Rapgef6) gene knockout mice. Exon 21 was removed. Homozygous knockout mice are viable and fertile, but exhibit an inlarged spleen, increased IgE and IgG levels and altered cytokine production.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes.
Yoshikawa Y, Satoh T, Tamura T, Wei P, Bilasy S E, Edamatsu H, Aiba A, Katagiri K, Kinashi T, Nakao K, Kataoka T
Mol. Biol. Cell, 18, 2949-2959 (2007). 17538012

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rapgef6
MGI:2384761		Rap guanine nucleotide exchange factor (GEF) 611Rapgef6<tm1.1Tkat>
MGI:3760543		targeted mutation 1.1, Tohru Kataoka
loxP phage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell adhesion(MP:0003566)

  • abnormal seminiferous tubule morphology(MP:0002216)

  • abnormal sperm head morphology(MP:0009230)

  • abnormal spermatogenesis(MP:0001156)

  • asthenozoospermia(MP:0002675)
    • more 6 phenotypes
  • enlarged spleen(MP:0000691)

  • homeostasis/metabolism phenotype(MP:0005376)

  • oligozoospermia(MP:0002687)

  • reduced male fertility(MP:0001922)

  • small testis(MP:0001147)

  • testicular atrophy(MP:0003205)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Rapgef6 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell, 18, 2949-2959 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTohru Kataoka (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Levy RJ, Kvajo M, Li Y, Tsvetkov E, Dong W, Yoshikawa Y, Kataoka T, Bolshakov VY, Karayiorgou M, Gogos JA.
    Deletion of Rapgef6, a candidate schizophrenia susceptibility gene, disrupts amygdala function in mice.
    Transl Psychiatry 5(6) e577(2015) 26057047