Strain Data Sheet

RBRC02716

Strain Information

Image
BRC No.RBRC02716
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Plce1<tm1Tkat>
Former Common namePLCεKO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. T. Kataoka, N. Suzuki and H. Saito in 2001.
Strain descriptionPhospholipase Cepsilon is a novel class of phosphoinositide-specific phospholipase C that were identified as a downstream effector of Ras and Rap small GTPases. This knockout strain expesses a phospolipase Cepsilon catalytically inactivated by gene targeting. Homozygous mutant mice show a congenital semilunar valvulogenesis defect.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Crucial role of phospholipase Cepsilon in chemical carcinogen-induced skin tumor development.
Bai Y, Edamatsu H, Maeda S, Saito H, Suzuki N, Satoh T, Kataoka T
Cancer Res., 15, 8808-8810 (2004). 15604236

Congenital semilunar valvulogenesis defect in mice deficient in phospholipase C epsilon.
Tadano M, Edamatsu H, Minamisawa S, Yokoyama U, Ishikawa Y, Suzuki N, Saito H, Wu D, Masago-Toda M, Yamawaki-Kataoka Y, Setsu T, Terashima T, Maeda S, Satoh T, Kataoka T
Mol. Cell Biol., 25, 2191-2199 (2005). 15743817

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Plce1
MGI:1921305		phospholipase C, epsilon 119Plce1<tm1Tkat>
MGI:3525208		targeted mutation 1, Tohru Kataoka
  • nephrotic syndrome, type 3(MedGEN)
    • neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal semilunar valve morphology(MP:0002746)

  • aortic valve regurgitation(MP:0006047)

  • aortic valve stenosis(MP:0006117)

  • bicuspid aortic valve(MP:0010484)

  • decreased cardiac muscle contractility(MP:0005140)
    • more 8 phenotypes
  • decreased incidence of tumors by chemical induction(MP:0004502)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • dilated heart left ventricle(MP:0002753)

  • dilated heart right ventricle(MP:0002754)

  • enlarged heart(MP:0000274)

  • pulmonary valve regurgitation(MP:0006048)

  • thick aortic valve(MP:0010594)

  • thick pulmonary valve(MP:0010601)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 late gene polyA signal, mouse Plce1 genomic DNA
    Research applicationCardiovascular Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 25, 2191-2199 (2005). Cancer Res., 64, 8808-8810 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTohru Kataoka (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data