Strain Data Sheet

RBRC02717

Strain Information

Image
BRC No.RBRC02717
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-xstopx-Plce1,-lacZ)ATkat
Former Common nameCAG-flox-PLCε-A
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Overexpression of phospholipase Cepsilon in keratinocytes upregulates cytokine expression and causes dermatitis with acanthosis and T-cell infiltration.
Takenaka N, Edamatsu H, Suzuki N, Saito H, Inoue Y, Oka M, Hu L, Kataoka T
Eur J Immunol., 41(1):202-213(2011). 21182091

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)UN
  • nephrotic syndrome, type 3(MedGEN)
  • Plce1phospholipase C, epsilon 1 cDNAUNPlce1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • nephrotic syndrome, type 3(MedGEN)
  • lacZbeta-galactosidase (E. coli)UN
  • nephrotic syndrome, type 3(MedGEN)
  • loxPphage P1 loxPUNloxP
  • nephrotic syndrome, type 3(MedGEN)
  • loxPphage P1 loxPUNloxP
  • nephrotic syndrome, type 3(MedGEN)
  • poly ASV40 polyA signalUNpoly A
  • nephrotic syndrome, type 3(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cytokine secretion(MP:0003009)

  • acanthosis(MP:0001874)

  • hyperkeratosis(MP:0001242)

  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • increased interleukin-1 alpha secretion(MP:0008654)
  • more 5 phenotypes
  • increased interleukin-1 beta secretion(MP:0008657)

  • increased interleukin-23 secretion(MP:0008690)

  • parakeratosis(MP:0003427)

  • skin hyperplasia(MP:0001214)

  • skin inflammation(MP:0004947)
  • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, STOP sequence contained a 550-bp C-terminal sequence of yeast His3 gene, mouse Plce1 cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. European Journal of Immunology, 41, 202-213 (2011).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTohru Kataoka (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data