Strain Data Sheet

RBRC02725

Strain Information

Image
BRC No.RBRC02725
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gpr89<tm1>
Former Common nameGphrloxp<+/->
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
GPHR-dependent functions of the Golgi apparatus are essential for the formation of lamellar granules and the skin barrier.
Tarutani M, Nakajima K, Uchida Y, Takaishi M, Goto-Inoue N, Ikawa M, Setou M, Kinoshita T, Elias P M, Sano S, Maeda Y
J. Invest. Dermatol. 132(8):2019-25 (2012). 22572823

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
Gpr89
MGI:1914799		G protein-coupled receptor 893Gpr89<tm1Ymae> targeted mutation 1, Yusuke Maeda
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal ear lobe morphology(MP:0003677)

  • abnormal epidermis stratum basale morphology(MP:0001231)

  • abnormal hair follicle morphology(MP:0000377)

  • abnormal keratinocyte morphology(MP:0002655)

  • abnormal reproductive system development(MP:0003936)
    • more 11 phenotypes
  • abnormal skin development(MP:0003941)

  • alopecia(MP:0000414)

  • decreased body weight(MP:0001262)

  • dermatitis(MP:0001194)

  • enlarged sebaceous gland(MP:0000652)

  • hyperkeratosis(MP:0001242)

  • hypopigmentation(MP:0005408)

  • impaired skin barrier function(MP:0002796)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • scaly skin(MP:0001192)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Yeast FRT (flipase recombinant target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Mouse Gpr89 genomic DNA, [CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), yeast FLPe cDNA]
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 132, 2019-2025 (2012). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use.
    DepositorTaroh Kinoshita (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data