Strain Information | |
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Image | ![]() |
BRC No. | RBRC02760 |
Type | Targeted Mutation Congenic![]() |
Species | Mus musculus |
Strain name | B6.Cg-Atg7<tm1.1Tchi> |
Former Common name | C57BL/6-Atg7+/- |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Komatsu and Chiba, Tokyo Metropolitan Institute of Medical Science. |
Strain description | Atg7, autophagy-related 7 gene knock-out mice. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Homozygous mutant mice die within 1 day of delivery. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl]Homozygous mutant mice die within 1 day after birth. |
References | Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T J. Cell Biol., 169, 425-434 (2005). 15866887 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Atg7 MGI:1921494 | autophagy related 7 | 6 | Atg7<tm1.1Tchi> MGI:3587771 | targeted mutation 1.1, Tomoki Chiba | |||
loxP | phage P1 loxP | 6 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, Mouse Atg7 genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Blol., 169, 425-434 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masaaki Komatsu (Tokyo Metropolitan Institute of Medical Science) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2012, Mouse of the Month Oct 2016 |
BRC mice in Publications |
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Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 140(3) 313-26(2010) 20144757 |