Strain Data Sheet

RBRC02761

Strain Information

Image
BRC No.RBRC02761
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Atg3<tm1Kmts>/TchiRbrc
Former Common nameC57BL/6-Atg3+/-, B6.Cg-Atg3<tm1>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice.
Sou Y S, Waguri S, Iwata J, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M
Mol. Biol. Cell., 19(11):4762-4775 (2008). 18768753

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atg3
MGI:1915091		autophagy related 316Atg3<tm1Kmts>
MGI:5609520		targeted mutation 1, Masaaki Komatsu
neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal autophagy(MP:0008260)

  • abnormal circulating amino acid level(MP:0005311)

  • decreased birth weight(MP:0009674)

  • increased susceptibility to induced morbidity/mortality(MP:0009763)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPolyoma enhancer/herpes simplex virus thymidine kinase gene (MC1) promoter, E. coli Neomycin resistance gene, Mouse Atg3 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell, 19, 4762-4775 (2008). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasaaki Komatsu (Juntendo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Mizushima N, Yoshimori T, Levine B.
    Methods in mammalian autophagy research.
    Cell 140(3) 313-26(2010) 20144757