Strain Data Sheet

RBRC02781

Strain Information

Image
BRC No.RBRC02781
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cd8a<a> Tg(Tcra2C,Tcrb2C)1Dlo
Former Common name2C TCR, B6-Cd8a<a> 2CTCR Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe transgene was developed by Dennis Loh, Washington University School of Medicine in 1988. The mice were deposited by Takanori Teshima, Kyushu University Hospital, through Paul Martin, Fred Hutchinson Cancer Research Center. H-2b and Ly2.1 (Cd8.1) background.
Strain description2C TCR transgenic mice expressing the alpha beta antigen receptor from the cytotoxic T-lymphocyte clone 2C. Ly2.1 (Cd8.1) congenic line.
Colony maintenanceTg : Carrier x Noncarrier ; Ly2 : Homozygote x Homozygote
References
Selective expression of an antigen receptor on CD8-bearing T lymphocytes in transgenic mice.
Sha W C, Nelson C A, Newberry R D, Kranz D M, Russell J H, Loh D Y
Nature, 335, 271-274 (1988). 3261843

Impaired signaling in alloantigen-specific CD8+ T cells tolerized in vivo: employing a model of Ld-specific TCR transgenic mice transplanted with allogenic hearts under the cover of a short-term rapamycin treatment.
Chen H, Luo H, Xu D, Loh D Y, Daloze P M, Veillette A, Qi S, Wu J
J. Immunol., 157, 4297-4308 (1996). 8906803

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd8a
MGI:88346		CD8 subunit alpha6Cd8a<a> a variant
  • susceptibility to respiratory infections associated with CD8alpha chain mutation(MedGEN)
    • Tcra2C
    MGI:3621454    		T cell receptor alpha chainUNTcra2C
    MGI:3621454    		transgene insertion 1, Dennis Y Loh
    Tcrb2C
    MGI:3621454    		T cell receptor beta chainUNTcrb2C
    MGI:3621454    		transgene insertion 1, Dennis Y Loh
    neo neomycin resistance gene (E. coli)UN

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal CD8-positive, alpha beta T cell morphology(MP:0005010)

  • abnormal dermis papillary layer morphology(MP:0009545)

  • abnormal memory T cell physiology(MP:0008051)

  • decreased lymphocyte cell number(MP:0005016)

  • decreased thymocyte number(MP:0000715)
    • more 3 phenotypes
  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • seborrheic dermatitis(MP:0009792)

  • thick epidermis(MP:0001219)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Functional rearranged TCR VJ alpha and VDJ beta genes from a Lambda Dash library of 2C DNA, mouse Cosmid C41/45 from Balb/c, mouse Cosmid containing C alpha complementary DNA from DBA/2 liver cosmid library, E. coli neo
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorTakanori Teshima (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data