Strain Data Sheet

RBRC02794

Strain Information

Image
BRC No.RBRC02794
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Vps52<tm1Kab>/95Rbrc
Former Common nameVps52 #95(Acc.No.CDB0767K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain descriptionVps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K
Cell Rep., 2(5) 1363-74 (2012). 23142660

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site17Frt
Frt yeast FRT (flippase recombination target) site17Frt
HBB2 rabbit beta-globin17HBB2
Vps52
MGI:1330304		VPS52 GARP complex subunit17Vps52<tm1Kab>
MGI:6147870		targeted mutation 1, Kuniya Abe
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality, complete penetrance(MP:0011092)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK 3’end, rabbit beta-globin 3' end, mouse Vps52 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data