Strain Data Sheet

RBRC02821

Strain Information

Image
BRC No.RBRC02821
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gab2<tm1Thir>
Former Common nameGab2 KO mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6 and 129 mixed
Appearanceback or agouti (not fixed yet)
Strain developmentDeveloped by Toshio Hirano, Osaka University Graduate School of Medicine in 2002. R1 ES cells were used. C57BL/6 and 129 mixed background.
Strain descriptionGab2 gene knockout mice. Exon encoding a major portion of the PH domain was replaced with a neo cassette. Gab2 gene is an adaptor molecule that plays a critical role in mediating cellular transformation. The number of mast cells is severely reduced in the stomach and less severely in the skin of homozygous mutant mice. Bone marrow-derived mast cells from the homozygous mutant mice exhibit an impaired KitL-induced ERK MAP kinase and Akt activation.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6JJcl] Reproductive performance is not so good.
References
Requirement of Gab2 for mast cell development and KitL/c-Kit signaling.
Nishida K, Wang L, Morii E, Park S J, Narimatsu M, Itoh S, Yamasaki S, Fujishima M, Ishihara K, Hibi M, Kitamura Y, Hirano T
Blood, 99 1866-1869 (2002). 11861309

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gab2
MGI:1333854		growth factor receptor bound protein 2-associated protein 27Gab2<tm1Thir>
MGI:2660754		targeted mutation 1, Toshio Hirano
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mast cell differentiation(MP:0005462)

  • abnormal mast cell physiology(MP:0002423)

  • decreased mast cell number(MP:0000336)

  • hematopoietic system phenotype(MP:0005397)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Gab2 genomic DNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 99, 1866-1869 (2002).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECPIENT who wants to use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University.
    DepositorToshio Hirano (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data