Strain Data Sheet

RBRC02829

Strain Information

Image
BRC No.RBRC02829
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.B6C3-Tg(Th-SNCA*130m)1702Yosh
Former Common nameSyn130m-1702, B6.Cg-Tg(Th-SNCA*)1702Yosh
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masaki Wakamatsu, Makoto Yoshimoto, Taisho Pharmaceutical Co., Ltd. in 2001. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mutant mice were backcrossed C57BL/6.
Strain descriptionTh-SCNA transgenic mice expressing human alpha synuclein under the control of tyrosine hydroxylase promoter. Syn130m line (RBRC02829) having a human alpha synuclein sequence with an Ala53Thr point mutation and a lacking of 10 amino acids at the C-terminus, Syn140m line (RBRC02830) having a human alpa synuclein sequence with an Ala 53Thr point mutation. Syn130m line (RBRC02829) is useful as a model of Parkinson Disease. Syn130m Tg mice exhibit decreased dopamine level and dopaminergic neuron number. Motor activity and exploratory behavior are reduced compared to controls. Tg homozygous mice are postnatal lethal. Syn140m line (RBRC02830) is available as a control of Syn130m line. Syn140m Tg mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Selective loss of nigral dopamine neurons induced by overexpression of truncated human alpha-synuclein in mice.
Wakamatsu M, Ishii A, Iwata S, Sakagami J, Ukai Y, Ono M, Kanbe D, Muramatsu S, Kobayashi K, Iwatsubo T, Yoshimoto M
Neurobiol. Aging, 574-585 (2008). 17174013

Dopamine receptor agonists reverse behavioral abnormalities of alpha-synuclein transgenic mouse, a new model of Parkinson's disease.
Wakamatsu M, Iwata S, Funakoshi T, Yoshimoto M
Neurosci. Res., 86. 640-646 (2008). 17896793

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin intronUNHBB2
SNCA synuclein, alpha (human)UNSNCA rat tyrosine hydroxylase (TH) promoter
SV40 polyA additional signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal neurite morphology(MP:0008415)

  • apnea(MP:0001957)

  • bradykinesia(MP:0005156)

  • decreased dopamine level(MP:0005643)

  • decreased dopaminergic neuron number(MP:0011448)
    • more 2 phenotypes
  • loss of dopaminergic neurons(MP:0003244)

  • neonatal lethality, complete penetrance(MP:0011087)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman mutated α-synuclein cDNA (A53T and a stop codon after amino acid 130), Rat Tyrosine hydroxylase promoter, Rabbit β-globin intron, SV40 polyA-additional signal
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurobiol. Aging, 29, 574-585 (2008).
    DepositorTaisho Pharmaceutical Co.,Ltd. (Taisho Pharmaceutical Co., Ltd.)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data