Strain Data Sheet

RBRC02831

Strain Information

Image
BRC No.RBRC02831
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Gcg<tm1Yhys>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Yoshitaka Hayashi, Nagoya University in 2007. Gcg gene was disrupted by introduction of GFP cDNA. GFP expresses in Gcg positive cells.
Strain descriptionGlucagon, Gcg deficient mice. Homozygous mutant mice lack peptides derived from proglucagon.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Mice deficient for glucagon gene-derived peptides display normoglycemia and hyperplasia of islet {alpha}-cells but not of intestinal L-cells.
Hayashi Y, Yamamoto M, Mizoguchi H, Watanabe C, Ito R, Yamamoto S, Sun X Y, Murata Y
Mol. Endocrinol., 23, 1990-1999 (2009). 19819987

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)2
Gcg
MGI:95674		glucagon2Gcg<tm1Yhys> transgene insertion 1, Yoshitaka Hayashi
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal pancreatic alpha cell morphology(MP:0005216)

  • decreased circulating insulin level(MP:0002727)

  • digestive/alimentary phenotype(MP:0005381)

  • improved glucose tolerance(MP:0005292)

  • increased body length(MP:0001257)
    • more 8 phenotypes
  • increased body weight(MP:0001260)

  • increased circulating glucose level(MP:0005559)

  • increased insulin sensitivity(MP:0002891)

  • increased pancreas weight(MP:0009108)

  • increased pancreatic islet number(MP:0009167)

  • polyuria(MP:0001762)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAA. victoria Enhanced green fluorescent protein cDNA, Bovine Growth hormone poly adenylation signal, mouse phosphoglycerate kinase promoter, E. coli Aminoglycoside phosphotransferase cDNA (E.coli neo), mouse phosphoglycerate kinase poly adenylation signal, mouse Gcg genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoshitaka Hayashi (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2012

    BRC mice in Publications

    Serrano J, Meshram NN, Soundarapandian MM, Smith KR, Mason C, Brown IS, Tyrberg B, Kyriazis GA.
    Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis.
    Biomedicines 10(1) (2022) 35052799
    Fukami A, Seino Y, Ozaki N, Yamamoto M, Sugiyama C, Sakamoto-Miura E, Himeno T, Takagishi Y, Tsunekawa S, Ali S, Drucker DJ, Murata Y, Seino Y, Oiso Y, Hayashi Y.
    Ectopic expression of GIP in pancreatic β-cells maintains enhanced insulin secretion in mice with complete absence of proglucagon-derived peptides.
    Diabetes 62(2) 510-8(2013) 23099862