Strain Data Sheet

RBRC02838

Strain Information

Image
BRC No.RBRC02838
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-GFP/DA)15
Former Common nameGFP-DATg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Tomoaki Shirao, Gunma University School of Medicine in 2001.
Strain descriptionDrebrin is an F-actin-binding protein that is highly expressed in the brain and regulates the dynamics of actin cytoskeletal networks. These mice specifically overexpress a fusion protein of rat drebrin A and GFP in the forebrain. A western blot analysis of cerebral cortex extract revealed a 10 to 15% increase in drebrin (similar expression patterns were observed in three lines: RBRC02838, RBRC02839, RBRC0284015). These mice are useful for analyzing the effect of the overexpression of drebrin at the cellular and body levels.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dbn1 drebrin 1 (rat)UNDbn1
GFP Green Fluorescent Protein (Aequorea victoria)UN mouse Camk2a promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal actin cytoskeleton morphology(MP:0020849)

  • abnormal dendrite morphology(MP:0008143)

  • abnormal dendritic spine morphology(MP:0009936)

  • abnormal dendritic thin spine morphology(MP:0020514)

  • abnormal microtubule cytoskeleton morphology(MP:0020850)
    • more 2 phenotypes
  • abnormal postsynaptic density morphology(MP:0020551)

  • increased dendritic spine length(MP:0020510)
    • Detailed phenotype data

    Ordering Information

    Donor DNAオワンクラゲGFP cDNA, ラット drebrin A cDNA, SV40 polyA signal, mouse Camk2a genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR/DEVELOPER to obtain permission for use o the BIOLOGICAL RESOURCE as a collaboration research.
    DepositorTomoaki Shirao (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data