Strain Data Sheet

RBRC02847

Strain Information

Image
BRC No.RBRC02847
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6/SvEvTac-Dkk3<tm1Tfur>
Former Common nameDKK3 K.O.
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain129S6/SvEvTac
Appearancelight-bellied agouti [A<w>/A<w> B/B C/C]
Strain developmentDeveloped by Dr. Takahisa Furukawa, Osaka Bioscience Institute in 2007.
Strain descriptionDkk3 knockout mice. Homozygous mutant mice show no obvious phenotype.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dkk3
MGI:1354952		dickkopf WNT signaling pathway inhibitor 37Dkk3<tm1Tfur>
MGI:5645089		targeted mutation 1, Takahisa Furukawa
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 pA, mouse Dkk3 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H.
    Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla.
    Cell Tissue Res 379(1) 157-167(2020) 31673758
    Inoue J, Fujita H, Bando T, Kondo Y, Kumon H, Ohuchi H.
    Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse.
    J Mol Histol 48(1) 29-39(2017) 27866302
    Zhu Y, Demidov ON, Goh AM, Virshup DM, Lane DP, Bulavin DV.
    Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.
    J Clin Invest 124(7) 3263-73(2014) 24911145