Strain Data Sheet

RBRC02849

Strain Information

Image
BRC No.RBRC02849
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ern1<tm1Dron>
Former Common nameIrel alpa knockout mouse +/- strain:129/C57BL/Black Swiss Webster
H-2 Haplotype
ES Cell lineW4 [129S6/SvEvTac]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. David Ron, New York University School of Medicine. Gene targeting was achieved in W4 ES cells.
Strain descriptionErn1, endoplasmic reticulum (ER) to nucleus signaling 1 gene knock-out mice. Homozygous Ern1 mutant mice are embryonic lethal during organogenesis.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]Homozygotes are embryonic lethal.
References
Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1.
Urano F, Wang X, Bertolotti A, Zhang Y, Chung P, Harding H P, Ron D
Science, 287, 664-666 (2000). 10650002

IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA.
Calfon M, Zeng H, Urano F, Till J H, Hubbard S R, Harding H P, Clark S G, Ron D
Nature, 415, 92-96 (2002). 11780124

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ern1
MGI:1930134		endoplasmic reticulum to nucleus signalling 111Ern1<tm1Dron> targeted mutation 1, David Ron
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal glucose homeostasis(MP:0002078)

  • abnormal response/metabolism to endogenous compounds(MP:0003638)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Ern1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin phosphotransferase
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 287, 664-666 (2000). Nature, 415, 92-96 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. DEPOSITOR hereby excludes any and all warranties, implied or express, including warranties of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE or of non-infringement of patents or other proprietary right. The sample of BIOLOGICAL RESOURCE is provided "as is". In no event shall DEPOSITOR be liable for direct, indirect, special, incidental or consequential damages in connection with the BIOLOGICAL RESOURCE. RECIPIENT and RIKEN BRC will indemnify and hold DEPOSITOR harmless from any claims or liability resulting from the use of the BIOLOGICAL RESOURCE by you. Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent form the DEPOSITOR for such application.
    DepositorDavid Ron (New York University School of Medicine)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data