Strain Data Sheet

RBRC02866

Strain Information

Image
BRC No.RBRC02866
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAT-RhoK DN)3-3Koba
Former Common nameCAT-RhoA-K DN/3-3
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine at 1998. The transgene containing CMV promoter and bovine Rho-A DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain descriptionTransgenic mice conditionally expressed a dominant-negative form for RhoK by using the Cre-loxP system. The Rho GTPase is known to regulate diverse cellular processes, including cell morphology, cell motility, adhesion, and apoptosis. The activity of RhoK in these transgenic mice is suppressed in developing motor neurons by crossing with dopamine beta-hydroxylase promotor (DBH)-cre transgenic mice. This strain are useful for elucidation of the role of the Rho sigaling pathway in various tissues with tissue-specific cre Tg mice.
Colony maintenance
References
Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase.
Kobayashi K, Takahashi M, Matsushita N, Miyazaki J, Koike M, Yaginuma H, Osumi N, Kaibuchi K, Kobayashi K
J. Neurosci., 24, 3480-3488 (2004). 15071095

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
RhoK ras homolog gene family, member A (bovine)UNRhoK
cat E. coli chloramphenicol acetyltransferase (CAT)UN CMV promoter
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
myc-tag myc-epitope tagUNmyc-tag
poly A SV40 polyA signalUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACytomegalovirus CMV promoter, E. coli Chloramphenicol acetyl transferase, bovine Rho-kinase, SV40 polyA signal
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 24, 3480-3488 (2004).
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data